https://doi.org/10.1140/epjd/e2016-70274-6
Regular Article
Correlation between helium atmospheric pressure plasma jet (APPJ) variables and plasma induced DNA damage*
Radiation Laboratory and Department of Physics,
University of Notre Dame, Notre
Dame, IN
46556,
USA
a
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Received:
17
April
2016
Received in final form:
22
June
2016
Published online:
6
September
2016
Abstract
A helium atmospheric pressure plasma jet (APPJ) source with a dielectric capillary and two tubular electrodes was used to induce damage in aqueous plasmid DNA. The fraction of different types of DNA damage (i.e., intact or undamaged, double strand breaks (DSBs), and single strand breaks (SSBs)) that occurred as the result of plasma irradiation was quantified through analysis of agarose gel electrophoresis images. The total DNA damage increased with an increase in both flow rate and duration of irradiation, but decreased with an increase in distance between the APPJ and sample. The average power of the plasma was calculated and the length of APPJ was measured for various flow rates and voltages applied. The possible effects of plasma power and reactive species on DNA damage are discussed.
Contribution to the Topical Issue “Low-Energy Interactions related to Atmospheric and Extreme Conditions”, edited by S. Ptasinska, M. Smialek-Telega, A. Milosavljevic, B. Sivaraman.
© EDP Sciences, Società Italiana di Fisica, Springer-Verlag 2016
