https://doi.org/10.1140/epjd/e2017-80073-2
Regular Article
γH2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity – preliminary methodological study and discussion*
1 Department of Cell Biology and Radiobiology, Institute of Biophysics of CAS, 61265 Brno, Czech Republic
2 Department of Otorhinolaryngology and Head and Neck Surgery, St. Anne’s University Hospital and Faculty of Medicine, Masaryk University, 65691 Brno, Czech Republic
3 Department of Pathological Physiology, Faculty of Medicine, Masaryk University, 62500 Brno, Czech Republic
4 Department of Physiology, Faculty of Medicine, Masaryk University, 62500 Brno, Czech Republic
a
e-mail: falk@ibp.cz
Received: 31 January 2017
Received in final form: 10 July 2017
Published online: 19 September 2017
In order to improve patients’ post-treatment quality of life, a shift from surgery to non-surgical (chemo)radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of γH2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of γ-rays. To better understand complex tumor behavior, three different cell type primocultures – CD90−, CD90+, and a mixed culture of these cells – were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV–HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance.
© EDP Sciences, Società Italiana di Fisica, Springer-Verlag 2017
