Eur. Phys. J. D 16, 317-320 (2001)
https://doi.org/10.1007/s100530170119

Formation and manipulation of regular metallic nanoparticle arrays on bacterial surface layers: an advanced TEM study

¹ Institut für Werkstoffwissenschaft, Technische Universität Dresden, D-01069 Dresden, Germany
² Institut für Angewandte Physik (IAPD), Technische Universität Dresden, D-01069 Dresden, Germany

Corresponding author: ^a mertig@tmfs.mpgfk.tu-dresden.de

Received: 6 December 2000
Published online: 15 September 2001

Abstract

The template-directed formation of regular nanoparticle arrays on two-dimensional crystalline protein layers after their treatment with metal salt complexes was studied by transmission electron microscopy. For these investigations, bacterial surface layers (S layers), recrystallized in vitro into sheets and tube-shaped protein crystals with typical dimensions in the micrometer range, were used as the template. As identified by electron holography and scanning force microscopy, the S-layer tubes form alternating double layers when deposited onto a solid substrate surface. Two distinct pathways for the metal particle formation at the templates have been found: the site-specific growth of metal clusters by chemical reduction of the metal salt complexes, and the electron-beam induced growth of nanoparticles in the transmission electron microscope. Both mechanisms lead to regular arrays with particle densities . Nanoparticle formation by electron exposure takes exclusively place in the flat-lying double-layered protein tubes, where a sufficient amount of metal complexes can be accumulated during sample preparation.