https://doi.org/10.1007/s100530050254
NMR and pressure correlated analysis of metabolic changes in soft-X-rays irradiated yeast cells
1
Dipartimento di Scienza dei Materiali, Università di Milano, via Emanueli
15, 20126 Milano, Italy
2
Dipartimento di Biologia Animale, Università di Modena, via Berengario,
41100 Modena, Italy
3
Dipartimento di Fisica, Università di Modena, via Campi 21,
41100 Modena, Italy
4
Dipartimento di
Fisica, Università di Milano, via Celoria 16,
20133 Milano, Italy
5
Rutherford Appleton Laboratory, Central Laser Facility, Chilton,
Didcot, OX11 OQX Oxfordshire, UK
Received:
13
October
1998
Accepted:
30
October
1998
Published online: 15 February 1999
An NMR technique for the identification of the energy related molecules, AMP, ADP and ATP, is suitably taylored to follow up the dynamics of the glycolitic metabolism in a suspension of yeast cells. Such a technique proves to be efficient to investigate soft X-ray radiation damage as complementary to other physical and chemical assessment.
PACS: 87.64.Hd – EPR and NMR spectroscopy / 87.22.-q – Physics of cellular and physiological processes / 87.50.Gi – Ionizing radiations (ultraviolet, X-rays, γ-rays, ions, electrons, positrons, neutrons, and mesons, etc.)
© EDP Sciences, Società Italiana di Fisica, Springer-Verlag, 1999
